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超薄切片观察表明,在三种感染芜菁花叶病毒的榨菜叶肉细胞内均发现有各种形状的内含体和病毒粒子的存在。内含体形状有风轮状、束状、管状、环状和板状聚集体,且不同抗性的榨菜品种细胞内所含的内含体数量、种类及所占的比例亦不同,抗性较弱的90-149榨菜品种叶肉细胞内内含体数量较多,以管状及环状内含体所占比例为多,束状及风轮状内含体所占比例较少;抗性较强的90-139榨菜品种叶肉细胞内内含体数量较少,且以束状及风轮状内含体所占比例较多,环状及管状内含体所占比例较少。而抗性中等的90-146榨菜品种叶肉细胞内内含体数量及所占比例介于上述两者之间。病毒粒子也以易感病的90-149榨菜叶肉细胞内含量最多,均以束状或拟晶格状存在。此外感病初期细胞内线粒体及粗面内质网数量有不同程度的增多,且以易感病的品种叶肉细胞内这些细胞器增加明显,但健康植株内以抗性较强的品种含这些细胞器较多。随着病毒症状的发展,叶绿体畸形,肿大,基质消失,外膜破裂直至解体。
The observation of ultrathin sections showed that there were various shapes of inclusion bodies and virus particles in the cells of the mustard leaf cells of the three infected turnip mosaic virus. The shape of the inclusion body has the shape of wind wheel, bundle, tube, ring and plate-like aggregates, and the quantity, the type and the proportion of the inclusion bodies contained in the cells of different resistance mustard varieties are also different, and the resistance Weak 90-149 mustard varieties leaf mesophyll cells contained a larger number of tubular and annular inclusions in the proportion of more bundles and wind-shaped wheel-shaped inclusions accounted for less; resistance than The strong 90-139 tuber mustard cells contained fewer mesophyll cells, with a greater proportion of bundles and wind-like inclusions, with fewer annular and tubular inclusions. However, the number and proportion of mesophyll in 90-146 tuber mustard seed medium with intermediate resistance were between the above two. Virus particles also susceptible to disease 90-149 mustard mesophyll cells content, are bundles or lattice-like presence. In addition, early mitochondria and rough endoplasmic reticulum in the early stages of susceptibility increased in varying degrees, and susceptible varieties of mesophyll cells organelles increased significantly, but healthy plants with more resistant varieties containing these organelles many. With the development of virus symptoms, chloroplast deformity, enlargement, matrix disappeared, the outer membrane rupture until disintegration.