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目的:建立测定大籽獐牙菜中1-羟基-3,7,8-三甲氧基酮、1,5,8-三羟基-3-甲氧基酮、1,8-二羟基-3,7-二甲氧基酮、1,7-二羟基-3,8-二甲氧基酮4种酮类成分含量的HPLC方法。方法:采用Zorbax SB-C18色谱柱(4.6mm×250mm,5μm),以甲醇-水进行梯度洗脱(0min,65:35;50min,70:30;50.1min,65:35;60min,65:35);流速:1.0mL/min;检测波长:254nm;柱温:30℃。结果:1-羟基-3,7,8-三甲氧基酮、1,5,8-三羟基-3-甲氧基酮、1,8-二羟基-3,7-二甲氧基酮、1,7-二羟基-3,8-二甲氧基酮的线性范围分别为(0.0173-0.8640)μg,(0.0623-3.1200)μg,(0.0305-3.0500)μg和(0.0562-5.6200)μg,相关系数r分别为0.9997,0.9999,0.9998和0.9999,其平均回收率(n=5)分别为100.1%,99.7%,100.5%,99.7%,RSD分别为1.1%,1.3%,1.2%,1.5%。结论:本方法可用于大籽獐牙菜的质量控制。
Objective: To establish a method for the determination of 1-hydroxy-3,7,8-trimethoxy ketone, 1,5,8-trihydroxy-3-methoxy ketone, 1,8-dihydroxy- 7-dimethoxy ketone, 1,7-dihydroxy-3,8-dimethoxy ketone ketone content of 4 kinds of HPLC method. METHODS: A gradient was performed on a Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) with methanol-water (0 min, 65:35; 50 min, 70:30; 50.1 min, 65:35; 60 min, 35); Flow rate: 1.0 mL / min; Detection wavelength: 254 nm; Column temperature: 30 ° C. Results: 1-Hydroxy-3,7,8-trimethoxy ketone, 1,5,8-trihydroxy-3-methoxy ketone, 1,8-dihydroxy-3,7-dimethoxyketone, The linear range of 1,7-dihydroxy-3,8-dimethoxyketone was (0.0173-0.8640) μg, (0.0623-3.1200) μg, (0.0305-3.0500) μg and (0.0562-5.6200) μg, respectively The coefficients r were 0.9997, 0.9999, 0.9998 and 0.9999, respectively. The average recoveries (n = 5) were 100.1%, 99.7%, 100.5% and 99.7%, respectively. The RSDs were 1.1%, 1.3%, 1.2% and 1.5%, respectively. Conclusion: The method can be used for the quality control of Swertia indica.