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目的应用超声背向散射积分(IBS)技术评价二甲双胍对糖尿病大鼠肾脏纤维化的干预作用。方法 30只雄性Wistar大鼠,随机抽取20只高脂饲料喂养4周后,腹腔注射链脲佐菌素(STZ,40mg/kg)建立糖尿病大鼠模型。模型成功后,将其随机分成模型对照组(DM组)、二甲双胍干预组(MET组),余下10只作为正常对照组(NC组)。NC和DM组蒸馏水灌胃2ml/d,MET组二甲双胍0.5g.kg-1.d-1)灌胃。8周后,超声测量肾实质标化背向散射积分值(IBS%),对肾组织进行羟脯氨酸浓度(HPC)测定;PAS染色计算肾小球系膜基质指数(Ms/Gs),Masson染色观察肾小球胶原沉积评分(GCDS)、血管周围胶原面积(PVCA)、肾小管间质病变评分(TIDS)。结果与NC组比较,DM组IBS%及纤维化指标(HPC、Ms/Gs、GCDS、TIDS、PVCA)均明显增高(P<0.05);与DM组比较,MET组IBS%减低(P<0.05),纤维化指标减轻,但未达NC组水平。IBS%与纤维化指标呈正相关(P<0.05)。结论糖尿病大鼠存在明显的肾脏纤维化;二甲双胍可以在一定程度上减轻纤维化病变;超声IBS技术可以检测到糖尿病大鼠肾脏纤维化及二甲双胍的干预效果。
Objective To evaluate the effect of metformin on renal fibrosis in diabetic rats by using ultrasonic backscatter integration (IBS) technique. Methods Thirty male Wistar rats were randomly selected from 20 high fat diets for 4 weeks. After streptozotocin (STZ, 40 mg / kg) was intraperitoneally injected into rats, a diabetic rat model was established. After the success of the model, they were randomly divided into model control group (DM group), metformin intervention group (MET group) and the remaining 10 normal control group (NC group). The rats in NC and DM groups were given 2ml / d distilled water and metformin 0.5g.kg-1.d-1 in MET group. After 8 weeks, the integral value (IBS%) of renal parenchyma was measured by ultrasound, and the hydroxyproline concentration (HPC) of kidney was measured. The mesangial matrix index (Ms / Gs) Masson staining glomerular collagen deposition score (GCDS), perivascular collagen area (PVCA), tubulointerstitial lesions score (TIDS). Results IBS% and fibrosis indexes (HPC, Ms / Gs, GCDS, TIDS and PVCA) in DM group were significantly higher than those in NC group (P <0.05) ), Fibrosis indicators reduced, but not up to the NC group level. IBS% was positively correlated with fibrosis (P <0.05). Conclusions Diabetic rats have obvious renal fibrosis. Metformin can reduce fibrosis to a certain degree. Ultrasound IBS can detect the effect of renal fibrosis and metformin in diabetic rats.