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目的探讨shRNA沉默EZH2表达对人肺癌A549细胞增殖和侵袭能力的影响。方法利用RT-PCR方法检测EZH2在人肺癌细胞株中的表达,特异性shRNA沉默EZH2表达后分析NCI-A549细胞增殖和侵袭能力的变化。结果检测14株非小细胞肺癌细胞中EZH2的mRNA表达,80%的细胞株EZH2较正常肺组织高表达;shRNA阻断EZH2表达可显著降低人肺癌A549细胞的增殖和侵袭能力(P<0.05)。结论 EZH2基因沉默能有效抑制人肺癌细胞的增殖和侵袭能力,为深入研究EZH2在非小细胞肺癌中作用提供了研究基础。
Objective To investigate the effect of shRNA silencing EZH2 on the proliferation and invasion of human lung cancer cell line A549. Methods The expression of EZH2 in human lung cancer cell lines was detected by RT-PCR. The expression of EZH2 was detected by specific shRNA. The proliferation and invasion of NCI-A549 cells were analyzed. Results The mRNA expression of EZH2 in 14 non-small cell lung cancer cells was detected, and 80% of the EZH2 cells were highly expressed in normal lung tissues. ShRNA blockade of EZH2 expression significantly reduced the proliferation and invasion ability of human lung cancer A549 cells (P <0.05) . Conclusion EZH2 gene silencing can effectively inhibit the proliferation and invasion ability of human lung cancer cells and provide the basis for further study on the role of EZH2 in non-small cell lung cancer.