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目的建立南药“凉粉草”水溶性成分的HPLC指纹图谱,为药材质量控制提供依据。方法采用Kromasil C_(18)(4.6mm×250mm,5μm)色谱柱,乙腈-0.1%甲酸溶液梯度洗脱,流速1.0 mL·min~(-1),检测波长254nm,柱温25℃。结果建立凉粉草药材水溶性成分HPLC指纹图谱,确定11个共有色谱峰,并指认图谱中的5个色谱峰,分别为咖啡酸、异槲皮苷、迷迭香酸苷、紫云英苷、迷迭香酸。对16批凉粉草药材指纹图谱进行聚类分析和主成分分析,筛选并判断合格样品,以合格样品构建指纹图谱共有模式,并用国家药典委员会中药色谱指纹图谱相似度评价系统软件(2004B版)对其质量进行分析与评价。结论该色谱方法简单、准确、重复性好,可用于凉粉草药材的质量评价。
OBJECTIVE To establish HPLC fingerprinting of the water-soluble components of the southern medicine “GJ” to provide the basis for the quality control of medicinal materials. Methods The gradient elution was carried out on a Kromasil C 18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile-0.1% formic acid solution at a flow rate of 1.0 mL · min -1. The detection wavelength was 254 nm and the column temperature was 25 ℃. Results The HPLC fingerprints of water-soluble constituents of Grifola frondosa were established and 11 common chromatographic peaks were identified. Five chromatographic peaks were identified as caffeic acid, isoquercitrin, rosemary glycoside, Rosmarinic acid. Cluster analysis and principal component analysis (PCA) were performed on 16 batches of fingerprints of Grifola frondosa. Screening and judging eligibility samples were carried out to construct a common pattern of fingerprints with qualified samples. The software was used to evaluate the fingerprint similarity of Chinese traditional medicine by the State Pharmacopoeia Commission (2004B version) Its quality analysis and evaluation. Conclusion The chromatographic method is simple, accurate and reproducible. It can be used for the quality evaluation of jelly grass medicinal materials.