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根据非洲猪瘟病毒(ASFV)VP73蛋白的氨基酸序列推测抗原表位优势区域,合成多肽并与牛血清白蛋白(BSA)偶联,筛选有抗原性的多肽作为试纸条检测线的包被抗原,采用量子点作为标记材料对葡萄球菌蛋白A(SPA)进行标记,以抗SPA的多克隆抗体作为质控线的包被蛋白,制备快速检测ASFV抗体的量子点免疫层析试纸条。结果表明,所制备的试纸条与常见相关猪疫病阳性血清无交叉反应,与进口ELISA试剂盒对临床样品的检测符合率为100%;特异性强、敏感性高、稳定性好、操作简便,可用于ASFV抗体的快速检测和流行病学调查。
According to the deduced amino acid sequence of VP73 protein of African classical swine fever virus (ASFV), the polypeptide was synthesized and conjugated with bovine serum albumin (BSA). The antigenic polypeptide was screened for coating antigen Quantum dot was used to label staphylococcal protein A (SPA). Anti-SPA polyclonal antibody was used as the coating of the control line to prepare a quantum dot immunochromatographic strip for rapid detection of ASFV antibody. The results showed that there was no cross-reaction between the prepared test strip and the positive related swine positive sera, and the coincidence rate with imported ELISA kit was 100%. The specificity, sensitivity, stability and easy operation , Can be used for rapid detection of ASFV antibodies and epidemiological investigations.