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采集冬、夏季太原市大气细颗粒物(PM2.5),并制备PM2.5生理盐水混悬液.将35只雄性SD大鼠随机分为7组:对照组、3个不同剂量夏季PM2.5染毒组(0.2、0.6、1.5 mg·kg~(-1)体重)及3个不同剂量冬季PM2.5染毒组(0.3、1.5、2.7 mg·kg~(-1)体重),每组5只,气道滴注法染毒,每隔2 d染毒1次,共60 d.采用荧光实时定量PCR、Western blot、ELISA方法检测大鼠肺内质网应激指标——葡萄糖调节蛋白78(GRP78)、活化转录因子6(ATF6)、C/EBP同源蛋白(CHOP)、半胱氨酸天冬氨酸蛋白酶12(Caspase12)及血红素氧合酶-1(HO-1)的mRNA和蛋白表达变化.结果表明,与对照组相比,冬季中、高剂量PM2.5染毒组大鼠肺组织GRP78、ATF6、CHOP、Caspase12、HO-1 mRNA和蛋白表达显著增加,夏季高剂量PM2.5染毒组大鼠肺组织这5个基因mRNA和蛋白表达显著增加.冬季和夏季PM2.5组大鼠肺上述5个基因表达有剂量-效应关系.结果表明,太原市PM2.5亚慢性染毒可诱导大鼠肺内质网应激相关基因GRP78/ATF6/CHOP/HO-1表达,说明肺内质网应激反应加强;而CHOP和Caspase-12上调,提示与细胞凋亡关联的内质网相关性死亡途径被激活.冬季和夏季PM2.5引起内质网应激相关因子表达上调的效应没有显著差别.
Thirty-five male Sprague-Dawley rats were randomly divided into seven groups: control group, three different doses of summer PM2.5 (0.2,0.6,1.5 mg · kg -1 body weight) and 3 different doses of PM 2.5 poisoning group (0.3,1.5,2.7 mg · kg -1 body weight) in winter, each group 5, instillation of airway instillation, once every 2 d, a total of 60 d.The real-time quantitative PCR, Western blot and ELISA were used to detect the endoplasmic reticulum stress index - glucose regulated protein 78, GRP78, ATF6, CHOP, Caspase 12 and HO-1 The results showed that compared with the control group, the mRNA and protein expressions of GRP78, ATF6, CHOP, Caspase12 and HO-1 in lung tissue of PM2.5 group increased significantly in summer and high in summer, The mRNA and protein expressions of these five genes in the lung tissue of PM2.5-treated rats were significantly increased, and the expression of PM5.5 and PM2.5 in the PM2.5 group was dose-effect-dependent. 5 sub-chronic exposure can be Induced the endoplasmic reticulum stress-related gene GRP78 / ATF6 / CHOP / HO-1 expression in the rat lung, indicating that the stress response of the lung endoplasmic reticulum was strengthened; while the upregulation of CHOP and Caspase-12, suggesting the endoplasmic reticulum Related death pathways were activated, and there was no significant difference in the effect of PM2.5-induced up-regulation of endoplasmic reticulum stress-related factors in winter and summer.