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为了探讨重金属Cu对堆肥过程的影响,以猪粪、麦秸、废菌糠为原料并接种复合微生物菌剂,在静态堆肥条件下,研究了Cu对堆肥过程中温度、微生物群落代谢能力和水解酶活性的变化。结果表明,CK处理(不添加Cu)高温期维持5d(其中55℃以上维持4d)达到无害化的温度要求;添加Cu处理后,L处理(Cu浓度为100mg.kg-1)高温期(>50℃)只持续4d;H处理(Cu浓度为500mg.kg-1)在整个堆肥过程中只有1d超过55℃,高温期只维持2d,L、H处理均未达到无害化的温度要求。以Biolog方法为主要检测手段并结合聚类分析和主成分分析方法,分析了重金属Cu对堆肥过程中微生物群落代谢能力的影响,结果表明,低剂量Cu能提高微生物群落对聚合物类碳源的转化与利用的能力,高剂量Cu对微生物群落利用中间代谢物和复杂大分子类碳源产生一定的抑制作用。水解酶活性分析结果表明,低剂量Cu对水解酶有一定的激活效应,高剂量Cu对水解酶有一定的抑制效应。
In order to study the effect of heavy metal Cu on the composting process, pig manure, wheat straw and waste bran were used as raw materials and inoculated with compound microbial agents. Under static composting conditions, the effects of Cu on temperature, microbial community metabolic capacity and hydrolase Changes in activity. The results showed that the CK treatment (no addition of Cu) maintained the detoxification temperature for 5 days at the high temperature (55 ℃ for 4 days). After adding Cu, the L treatment (Cu concentration 100mg.kg-1) > 50 ℃) for only 4 days. H treatment (Cu concentration of 500mg.kg-1) was only 55 ℃ for 1d and only 2 days for high-temperature composting, and L and H treatment did not reach the detoxification temperature requirement . The Biolog method was used as the main detection method and the cluster analysis and principal component analysis methods were used to analyze the effect of heavy metal Cu on the microbial metabolic capacity during composting. The results showed that low dose Cu could improve the microbial community carbon source Transformation and utilization of high-dose Cu on the use of intermediate metabolites and macromolecules microbial community have some inhibitory effect. The analysis of hydrolase activity showed that low dose of Cu had certain activation effect on hydrolase and high dose of Cu had certain inhibitory effect on hydrolase.