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采用溶胶凝胶法在58S生物玻璃的基础上用氧化锌取代3 mol%的氧化钙制备了含锌的生物玻璃粉体(58S3Z),对合成的粉体采用有机泡沫浸渍法在700℃及1200℃制备出58S3Z-700℃、58S3Z-1200℃玻璃及玻璃陶瓷多孔支架。在所得支架表面涂覆PLGA及PBS薄膜制备出58S3Z-1200℃-PLGA及58S3Z-1200℃-PBS复合支架。对其形貌、孔隙率、力学性能、体外降解性及细胞相容性进行了系统研究。复合后多孔支架仍然保持三维连通的多孔结构,孔隙率与复合前(86.9%±0.8%(58S3Z-700℃),80.1%±0.6%(58S3Z-1200℃))相比稍有下降,分别为75.9%±0.6%(58S3Z-1200℃-PLGA)和77.9%±0.9%(58S3Z-1200℃-PBS)。但复合多孔支架显示出较高的抗压强度,分别达到1509.4 kPa±162.8 kPa(PLGA)和901.6 kPa±94.5 kPa(PBS),与玻璃和玻璃陶瓷支架(258.4 kPa±23.6 kPa)相比具有较大的提高。体外降解实验表明58S3Z-1200℃-PLGA、58S3Z-1200℃-PBS复合多孔支架可降解,经过28天的浸泡其失重率分别达到13.3%和2.1%。体外研究结果表明:58S3Z玻璃陶瓷支架复合PBS或PLGA后支持成骨细胞黏附、铺展和生长。这种新型的复合支架具有三维的网状多孔结构,良好的力学性能、降解性和细胞相容性,有望成为一种较理想的骨组织工程支架。
Zinc-containing bioglass powder (58S3Z) was prepared by the sol-gel method on the basis of 58S bioglass with zinc oxide instead of 3 mol% calcium oxide. The synthesized powder was impregnated with organic foam at 700 ℃ and 1200 ℃ prepared 58S3Z-700 ℃, 58S3Z-1200 ℃ glass and glass ceramic porous scaffold. The resulting scaffold coated with PLGA and PBS film prepared 58S3Z-1200 ℃ -PLGA and 58S3Z-1200 ℃-PBS composite scaffold. The morphology, porosity, mechanical properties, in vitro degradation and cell compatibility were systematically studied. The composite porous scaffold still maintained three-dimensional porous structure with a slight decrease in porosity compared with that before composites (86.9% ± 0.8% (58S3Z-700 ° C), 80.1% ± 0.6% (58S3Z-1200 ° C)), respectively 75.9% ± 0.6% (58S3Z-1200 ° C-PLGA) and 77.9% ± 0.9% (58S3Z-1200 ° C-PBS). However, the composite porous scaffolds exhibited higher compressive strength of 1509.4 kPa ± 162.8 kPa (PLGA) and 901.6 kPa ± 94.5 kPa (PBS), respectively, compared with glass and glass ceramic scaffolds (258.4 kPa ± 23.6 kPa) Great improvement. In vitro degradation experiments showed that 58S3Z-1200 ℃ -PLGA, 58S3Z-1200 ℃ -PBS composite porous scaffolds were degradable. After 28 days immersion, their weight loss rates reached 13.3% and 2.1%, respectively. In vitro studies showed that: 58S3Z glass ceramic stent composite PBS or PLGA support osteoblast adhesion, spreading and growth. The new composite scaffold with three-dimensional network of porous structure, good mechanical properties, degradability and cell compatibility, is expected to become a more ideal bone tissue engineering scaffold.