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目的为甲型副伤寒沙门菌感染性疾病提供快速、准确的诊断信息和迅速的直接药敏结果。方法用酶联免疫吸附技术(ELISA)对发热住院患者血清标本进行甲型副伤寒沙门菌鞭毛抗原检测,并与常规检测方法的鉴定结果比较。对阳性血培养作直接药敏试验并与常规药敏试验结果进行比较。结果常规检测方法鉴定为甲型副伤寒沙门菌作为对照,ELISA法的敏感性和特异性达93.6%和90.7%,这两种方法有着极显著联系,菌种符合率在88.7%左右。直接药敏试验与常规药敏试验结果比较无显著性差异。结论用ELISA法检测甲型副伤寒沙门菌感染,具有敏感性高、特异性强、简便、快速等特点。直接药敏试验至少可提前24h报告结果,为临床合理、及时使用抗生素提供依据。
Objective To provide rapid and accurate diagnostic information and prompt direct drug susceptibility test for Salmonella paratyphi A infectious diseases. Methods Serum samples of patients with fever were detected by enzyme-linked immunosorbent assay (ELISA) for detection of Salmonella paratyphi A flagellar antigens, and compared with the results of conventional detection methods. Positive blood culture for direct drug susceptibility testing and conventional susceptibility testing results were compared. Results The results showed that Salmonella Paratyphi A was used as a control. The sensitivity and specificity of ELISA were 93.6% and 90.7%, respectively. There was a significant correlation between these two methods. The coincidence rate was 88.7%. Direct drug susceptibility test and conventional susceptibility test results showed no significant difference. Conclusion The detection of Salmonella paratyphi A by ELISA has the characteristics of high sensitivity, specificity, simplicity, rapidity and so on. Direct drug susceptibility test at least 24h report the results in advance, for clinical reasonable, timely use of antibiotics provide the basis.