论文部分内容阅读
目的:探讨β2-肾上腺素能受体(β2-adrenoceptor,β2-AR)基因编码区16位点基因多态性与云南汉族老年人群慢性阻塞性肺疾病(COPD)合并原发性高血压(EH)的相关性。方法:用等位基因特异性聚合酶链反应(AS-PCR)技术检测180例研究对象的β2-AR基因编码区第16位点单核苷酸多态性(SNP)。结果:β2-AR基因16位点基因型和等位基因频率在COPD合并EH组、COPD未合并EH组和健康对照组间的分布差异无统计学意义(P>0.05)。但16位点GlyGly与ArgGly基因型的COPD,其FEV1%预计值低于ArgArg基因型的患者。在COPD合并EH组,Gly16等位基因位频率在各级高血压患者中的分布分别为18%、33%和50%,其在各级高血压之间的分布差异均有统计学意义(均P<0.05)。结论:在本研究中,β2-AR基因16位点基因多态性与云南汉族老年COPD合并EH的发病无相关性。但β2-AR基因16位点基因多态性与COPD肺通气功能的损害程度有关,携带Gly16等位基因的COPD患者肺通气功能更易受到损害。β2-AR基因16位点基因多态性与COPD合并EH的血压分级有关,Gly16等位基因是COPD合并EH血压增高的因素。
Objective: To investigate the relationship between polymorphism of β2-adrenoceptor (β2-AR) gene coding region 16 locus and chronic obstructive pulmonary disease (COPD) in Yunnan Han elderly patients with essential hypertension (EH ) Relevance. Methods: SNP in the coding region of β2-AR gene in 180 subjects was detected by allele-specific polymerase chain reaction (AS-PCR). Results: There was no significant difference in the genotype and allele frequencies of 16 genotypes and alleles in the COPD patients with EH and those without COPD and those with healthy controls (P> 0.05). However, COPD with 16-site GlyGly and ArgGly genotypes predicted a lower FEV1% than patients with ArgArg genotype. In COPD with EH group, the frequency of Gly16 allele was 18%, 33% and 50% in all kinds of hypertensive patients, and the distribution of Gly16 allele in all the patients with hypertension was statistically significant (both P <0.05). Conclusion: In this study, there was no correlation between polymorphism of β2-AR gene 16 locus and incidence of elderly COPD with EH in Yunnan Han population. However, the gene polymorphism of β2-AR gene at 16 locus is associated with the degree of impairment of pulmonary ventilation in COPD patients. COPD patients with Gly16 allele are more susceptible to pulmonary ventilation. The polymorphism of β2-AR gene 16 locus is related to the blood pressure classification of COPD with EH. The Gly16 allele is the cause of hypertension in COPD with EH.