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采用硅胶柱色谱、凝胶柱色谱、闪式反相C_(18)柱色谱以及反相HPLC等多种色谱技术和方法,从链霉菌CPCC 202950大米发酵产物中分离得到8个化合物,借助理化性质和波谱学数据分析,鉴定其结构为3-[(3’-氨基-3’-氧丙-1’-烯-2’-基)氧]苯甲酰胺(1),间位-羟基苯甲酰胺(2),leptosphaepin(3),5-methyluracil(4),feruloylamide(5),对羟基苯乙酰胺(6),vanillamide(7),环-(L-缬氨酸-L-丙氨酸)(8)。其中化合物1为1个新的苯甲酰胺类化合物,2为新的天然产物。化合物1~8对HIV-1蛋白酶均没有明显的抑制作用,对Hela,HepG2和U2OS 3株肿瘤细胞株也未显示出明显的毒性(IC_(50)>10μmol·L~(-1))。
Eight compounds were isolated from fermentation products of Streptomyces sp. CPCC 202950 by silica gel column chromatography, gel column chromatography, flash reversed phase C_ (18) column chromatography and reversed-phase HPLC and other chromatographic techniques. With the help of physicochemical properties And spectroscopic data analysis, the structure was identified as 3 - [(3’-amino-3’-oxoprop-1’-en-2’yl) oxy] benzamide (1) (2), leptosphaepin (3), 5-methyluracil (4), feruloylamide (5), p -hydroxyphenylacetamide (6), vanillamide )(8). Among them, compound 1 is a new benzamide compound and 2 is a new natural product. Compounds 1 to 8 showed no significant inhibitory effect on HIV-1 protease, nor did they show significant cytotoxicity against Hela, HepG2 and U2OS tumor cell lines (IC 50> 10 μmol·L -1).