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目的:研究益脑康对动脉粥样硬化(AS)及AS基础上的急性缺血性中风(AIS)SD大鼠脑组织病理及脑组织血管内皮生长因子(VEGF)表达的影响,以期部分阐述其作用机制。方法:将体重(180±20)g SD雄性大鼠115只随机分为A组(正常组)15只;B,C,D,E,F组共100只大鼠(其中C组为AS组、D组为复合模型组、B组为益脑康预防组、E组为益脑康治疗组、F组为立普妥治疗组),第8 d一次性VD3腹腔注射后开始给养高脂饲料复制AS模型,第65 d,除C组外均采用ET-1 MCA附近注射法复制AIS模型。第74 d,每组随机抽取3只检测脑组织病理情况及VEGF表达情况。结果:益脑康组脑组织VEGF免疫组化结果提示血管壁纤维和内皮细胞着色强度为++,强于立普妥组。结论:益脑康可能通过上调AIS大鼠脑组织VEGF表达而发挥对AIS大鼠脑组织的保护作用。
Objective: To study the effect of Yi Nao Kang on the pathological changes of brain tissue and the expression of vascular endothelial growth factor (VEGF) in brain of rats with acute ischemic apoplexy (AIS) based on atherosclerosis (AS) and AS. Its mechanism of action. METHODS: One hundred and fifteen male SD rats weighing (180±20) g were randomly divided into 15 groups in group A (normal group); 100 rats in groups B, C, D, E, and F (including group C in AS group). The group D was the compound model group, the group B was the Yinaokang preventive group, the E group was the Yinaikang group, and the group F was the Lipitor group). The 8th day of the single-dose VD3 was given after the intraperitoneal injection of high fat diet. The AS model was replicated. On the 65th day, except for group C, the AIS model was replicated by injection near the ET-1 MCA. On the 74th day, 3 rats in each group were randomly selected to detect pathological conditions and VEGF expression in the brain. Results: The results of VEGF immunohistochemistry in the brain of Yinaokang group indicated that the staining intensity of vascular wall and endothelial cells was ++, which was stronger than Lipitor group. Conclusion: Yinaokang may play a protective role in brain tissue of AIS rats by up-regulating the expression of VEGF in brain tissue of AIS rats.