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目的:应激效应在多种心脏疾病的发生、发展中发挥重要作用。本研究探讨在心脏移植后慢性排斥反应期,是否存在应激效应对心肌和冠状动脉的损伤。方法:采用Ono模型建立大鼠心脏移植,采用蛋白质组技术比较同基因移植与异基因移植后2周、8周左室心脏组织的蛋白质变化,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)分析获得肽质量指纹图谱,经Matrix Science查询软件搜索获得匹配的蛋白质。结果:异基因移植心脏在术后2周即出现血管内膜增厚[(2.07%±0.93%)vs.(27.58%±11.14%),P<0.01)],在术后8周更明显[(2.34%±1.06%)vs.(72.29%±20.57%),P<0.01)];蛋白质组学技术分析左室心肌,发现了45个差异表达的蛋白质;通过数据库鉴定得到37种蛋白质,其中9个与应激和损伤相关蛋白质,进一步使用Western印迹验证了其中的2个。结论:移植后心肌持续受到应激效应的损伤可能是大鼠心脏移植后冠状动脉病变发生发展的一个重要原因。
Objective: Stress effects play an important role in the occurrence and development of many heart diseases. This study explored the effects of stress on myocardial and coronary arteries during chronic rejection after heart transplantation. Methods: The cardiac allograft was established by Ono model. Proteomic analysis was used to compare the changes of left ventricular cardiac tissue proteins at 2 and 8 weeks after allotransplantation and allogeneic transplantation. MALDI-TOF -MS) analysis of peptide mass fingerprinting, Matrix Science query software search to obtain the matching protein. Results: Intimal hyperplasia was observed 2 weeks after operation in allograft recipients ([2.07% ± 0.93%] vs (27.58% ± 11.14%), P <0.01) (2.34% ± 1.06%) vs. (72.29% ± 20.57%), P <0.01). Proteomic analysis of left ventricular myocardium revealed 45 differentially expressed proteins. Of the 37 proteins identified by the database, Nine proteins related to stress and injury were further verified by Western blotting. CONCLUSION: The sustained stress-induced myocardial damage after transplantation may be an important reason for the development of coronary artery disease after cardiac transplantation in rats.