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本研究旨在探讨白花香莲解毒方抑制乙型肝炎细胞Hep G2.2.15增殖的机制。首先通过对于我院检验科27例乙肝患者的血清进行收集,检测在不同病毒拷贝数的血清中蛋白激酶2(MAP3K2)的含量,分析HBV DNA拷贝数与患者血清中MAP3K2含量的相关性;再通过细胞实验,在不同时间点(第3,5天)检测通过白花香莲解毒方处理后的Hep G2.2.15细胞上清中MAP3K2含量变化情况,以及细胞集落形成能力的差异。结果发现,患者血清中HBV DNA拷贝数与MAP3K2含量呈正相关,相关系数R~2=0.251;观察组细胞通过白花香莲解毒方处理后,细胞集落形成能力明显低于对照组,且第3、5天细胞检测上清液中MAP3K2含量均低于对照组,差异有统计学意义(p<0.05)。因此本研究认为,白花香莲解毒方是通过抑制MAP3K2的合成,从而达到控制乙型肝炎病毒活跃程度的目的,减少HBV DNA的合成。
The purpose of this study was to investigate the mechanism of Bahua Xianglianjiedu Decoction inhibiting the proliferation of Hep G2.2.15 cells. First of all, 27 cases of hepatitis B patients from our laboratory were collected for detection of protein kinase 2 (MAP3K2) in serum of different virus copy number, and the correlation between HBV DNA copy number and serum level of MAP3K2 Through cell experiment, we detected the content of MAP3K2 in the supernatant of Hep G2.2.15 cells treated with Baihua Xianglian detoxification at different time points (days 3 and 5), and the difference of cell colony-forming ability. The results showed that there was a positive correlation between the copy number of HBV DNA and the content of MAP3K2 in the serum of patients, the correlation coefficient was R ~ 2 = 0.251. After the cells in the observation group were treated with the extract of Radix Angelicae Sinensis, the colony forming ability was significantly lower than that of the control group, The contents of MAP3K2 in the supernatant of the 5-day cell assay were lower than those in the control group, with significant difference (p <0.05). Therefore, this study suggests that the Baihua Xianglian Jiedu Prescription can inhibit the synthesis of MAP3K2 so as to achieve the goal of controlling the activity of hepatitis B virus and reduce the synthesis of HBV DNA.