【摘 要】
：
To explore the effect of sudan Ⅰ, Ⅲ, and Ⅳ on the DNA/RNA ratio and changes in the 3D structure of HepG-2. LCM and 3D images are used to detect the DNA/RNA r
【机 构】
：
Center of Research and Development in Life Sciences and Environmental Sciences, Harbin University of
论文部分内容阅读
To explore the effect of sudan Ⅰ, Ⅲ, and Ⅳ on the DNA/RNA ratio and changes in the 3D structure of HepG-2. LCM and 3D images are used to detect the DNA/RNA ratio and changes in the 3D structure of HepG-2 when treated with different dosages of sudan Ⅰ, Ⅲ, and Ⅳ. The DNA/RNA ratio of the control group is 1. 223 2 ± 0. 084 4, while the fluorescence intensity of DNA in HepG-2 treated with sudan Ⅰ, Ⅲ, or Ⅳ is markedly greater than that of RNA, with the low-dosage group showing significant effect (P ＜ 0.01 ), yielding DNA/RNA ratios of 1. 609 6 ±0. 199 0, 1. 445 5 ±0. 163 3, 1. 708 1 ±0. 109 0 respectively; 3D images show that DNA fluorescence in HepG-2 is mostly concentrated in the nuclear region, and is denser and stronger than RNA fluorescence. The DNA/RNA ratio of a treated group increases after being treated with different dosages of sudan, but it declines with increasing dosage, and within a certain dosage range, sudan Ⅰ, Ⅲ, and Ⅳ are shown to promote the growth of HepG-2.
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