论文部分内容阅读
目的研究母体孕期及哺乳期骨骼铅动员的规律,并评价大剂量补充钙及VD3的干预效果。方法根据2×6析因设计原理,以补钙与否、孕期及哺乳期6个阶段分组,每组6只,将72只清洁级雌性昆明小鼠通过饮水暴露于乙酸铅(以铅计,50 mg/L),4周后与雄鼠同笼并受孕;铅暴露+钙及维生素D(3VD3)补充组母鼠在孕期及哺乳期内通过饮水补充碳酸钙(50 mg/L)及VD(30.42μg/L),单纯铅暴露组母鼠饮用蒸馏水,连续染毒42 d。分别在孕期的第1、7和14天,哺乳期的第1、10和21天取全血、股骨,采用石墨炉原子吸收法、火焰原子吸收法测定铅、钙含量。结果孕期及哺乳期不同阶段、补充钙及VD3与否均对母鼠血液、骨骼的铅、钙含量有影响(P=0.000),且两个因素之间存在着协同作用(P=0.000)。在整个观察期内,单纯铅暴露组母鼠的血钙、骨钙及骨铅水平持续下降(P<0.05),血铅水平持续上升(P<0.05);铅暴露+钙及VD3补充组母鼠的血钙、骨钙及骨铅水平相对稳定(P>0.05),血铅水平有所下降(P<0.05)。两组母鼠的血铅水平与血钙水平之间呈现负相关关系(r=-0.702,P<0.01),血铅水平与骨铅水平之间呈现负相关关系(r=-0.904,P<0.01)。结论铅染毒小鼠孕期及哺乳期存在着骨骼铅动员现象,并可被大剂量补充钙及VD3部分抑制。
Objective To study the regularity of skeletal lead mobilization during pregnancy and lactation in maternal and to evaluate the effect of high dose calcium supplementation and VD3 intervention. Methods According to the principle of 2 × 6 factorial design, 72 female mice of clean grade Kunming were divided into 6 groups by 6 stages of calcium supplement, pregnancy and lactation. 50 mg / L). After 4 weeks of gestation, males were housed in the same cage and pregnant. Lead exposure + calcium and vitamin D supplementation (3VD3) supplemented with calcium carbonate (50 mg / L) and VD (30.42μg / L), pure lead exposure group rats drinking distilled water, continuous exposure 42d. The blood and femur were collected on days 1, 10 and 21 of lactation on the first, seventh and fourteenth days of pregnancy respectively. The contents of lead and calcium were determined by graphite furnace atomic absorption spectrometry and flame atomic absorption spectrometry. Results Both calcium and VD3 at different stages of pregnancy and lactation had effects on the lead and calcium contents in the blood and bone of the female rats (P = 0.000), and there was a synergistic effect between the two factors (P = 0.000). During the whole observation period, blood calcium, bone calcium and bone lead levels in the lead exposed group continued to decline (P <0.05), blood lead levels continued to increase (P <0.05); lead exposure + calcium and VD3 supplementation group The levels of serum calcium, bone calcium and bone lead in the rats were relatively stable (P> 0.05), and the levels of blood lead decreased (P <0.05). There was a negative correlation between blood lead level and serum calcium level in the two groups (r = -0.702, P <0.01). There was a negative correlation between blood lead level and bone lead level (r = -0.904, P < 0.01). Conclusion Lead-induced mice have skeletal lead mobilization during pregnancy and lactation, and can be partially inhibited by high-dose calcium supplementation and VD3.