论文部分内容阅读
作者采用26天龄的大鼠,以10IU 孕马血清促性腺激素(PMSG)使卵巢卵泡生长同步后,处死大鼠并取出卵巢,在立体显微镜下分离出排卵前卵泡(每鼠10～15个),进行体外孵育。作者比较了孵育液中含有浓度为100ng/ml 的高活性 GnRH 类似物(GnRHa)和 LH 对孵育的卵泡甾体激素产生的影响。当孵育6小时时,与对照相比,含 GnRHa或 LH 的孵育液中卵泡产生甾体激素显著增多:孕烯醇酮增加分别为2和5.8倍;孕酮增加4和45倍;20α-羟-孕酮增加38和53倍;雄烯二醇增加3.6和
The authors used a 26-day-old rat to synchronize the growth of ovarian follicles with 10 IU pregnant horse serum gonadotropin (PMSG), and the rats were sacrificed and the ovaries removed. The anovulatory follicles were isolated under a stereomicroscope (10-15 mice per mouse ) For in vitro incubation. The authors compared the effect of high GnRH analogues (GnRHa) with 100 ng / ml in the incubation solution and LH on the production of FSH in the incubated follicles. When incubated for 6 hours, follicles producing steroid hormones were significantly increased in the incubation solution containing GnRHa or LH compared to the control: progesterone increased by 2 and 5.8 times, progesterone increased by 4 and 45 times, 20α-hydroxy - Progesterone increased by 38 and 53 times; Androstenediol increased by 3.6 and