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以硅基底和光纤纤芯阵列为定位夹具,手工排列熔凝法得到的Au微球,获得3×3 Au微球阵列;在Au微球表面先用水浴法合成ZnO纳米线、后固定葡萄糖氧化酶,得到球形跨尺度阵列葡萄糖酶电极。对球形跨尺度结构的表面形貌、葡萄糖氧化酶的固定效果、酶电极的电化学性能进行了定量表征。球形跨尺度结构阵列葡萄糖传感器的线性范围为0~2.5mmol/L、灵敏度为17.24μA·(mmol/L)~(-1)·cm~(-2)、最低检出限为3.44μmol/L、Michaelis-Menten常数为1.85 mmol/L。
Using a silicon substrate and an optical fiber core array as positioning fixtures, the Au microspheres obtained by the fusion method were manually arranged to obtain a 3 × 3 Au microsphere array. First, ZnO nanowires were synthesized by a water bath method on a surface of the Au microspheres and then fixed with glucose oxidase , To obtain spherical cross-scale array glucose enzyme electrode. The surface morphology of spherical cross-scale structure, the immobilization effect of glucose oxidase and the electrochemical performance of enzyme electrode were quantitatively characterized. The linear range of spherical cross-structured glucose sensor was 0-2.5mmol / L, the sensitivity was 17.24μA · mmol-1 · cm-2 and the minimum detection limit was 3.44μmol / L , Michaelis-Menten constant of 1.85 mmol / L.