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研究了 ＩＧＦ－ Ⅰ、 ＩＧＦ结合蛋白－２（ＩＧＦＢＰ－２）和促黄体激素受体（ＬＨＲ） ｍＲＮＡ在 卵泡闭锁过程中的表达及调节．给２６日龄大鼠注射 １５ ＩＵ ＰＭＳＧ，经检测，证实ＰＭＳＧ 处理 ４８ ｈ后，一些小窦状卵泡的颗粒细胞已发生凋亡； ９６ ｈ在排卵前卵泡中已可检测到 凋亡细胞； １２０ ｈ大多数的排卵前卵泡中均出现大量的凋亡细胞． ４８～ １２０ ｈ ＩＧＦ－ Ⅰ主要 在窦前卵泡和小窦状卵泡表达； ４８与 ９６ ｈ，窦前与窦状卵泡的膜细胞均表达高水平的 ＩＧＦＢＰ－２．在 ４８ ｈ，颗粒细胞中有 ＬＨＲ的强信号，但在 ９６和 １２０ ｈ，颗粒细胞的 ＬＨＲ表 达减弱（Ｐ＜０．００１）．表皮生长因子（ＥＧＦ）和ＩＧＦ－Ⅰ均抑制窦前和窦状卵泡颗粒细胞凋亡． 同时观察到 ＥＧＦ促进ＩＧＦ－ⅠｍＲＮＡ表达， ＩＧＦ－Ⅰ刺激排卵前卵泡表达ＬＨＲ ｍＲＮＡ．上 述结果表明，各级卵泡的闭锁可能均受ＥＧＦ和ＩＧＦ－Ⅰ相互作用的调节．
The expression and regulation of IGF-Ⅰ, IGFBP-2 and LHR mRNA during follicular atresia were studied. The 26-day-old rats were injected with 15 IU of PMSG. After 48 hours of PMSG treatment, granulosa cells of some small sinusoid follicles were apoptotic. Apoptotic cells could be detected in preovulatory follicles at 96 h Most ovarian follicles present a large number of apoptotic cells. At 48 to 120 h, IGF-I mainly expressed in preantral follicles and small sinusoid follicles; high levels of IGFBP-2 were expressed in membrane cells of anterior and sinusoidal follicles at 48 and 96 h. At 48 h, there was a strong signal of LHR in granulosa cells, but the LHR expression of granulosa cells was weakened at 96 and 120 h (P <0.001). Both epidermal growth factor (EGF) and IGF-I inhibit apoptosis of granulosa cells in anterior sinus and sinusoid follicles. At the same time, it was observed that EGF promoted IGF-ⅠmRNA expression, and IGF-Ⅰ stimulated LHR mRNA expression in preovulatory follicles. The above results indicate that atresia follicles at all levels may be regulated by the interaction of EGF and IGF-Ⅰ.