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目的研究α2β1整合素基因多态性与DN间的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP),对298例南京区T2DM患者(合并肾病182例,无肾病116例)和107名正常人进行α2β1Bg1Ⅱ基因多态性研究。结果 (1)DN组Bg1Ⅱ+/+基因型高于无肾病组(DM组)和正常对照(NC)组(P<0.01),与NC组比,DN组患者Bg1Ⅱ+等位基因频率增加(P<0.05);(2)T2DM患者Bg1Ⅱ+/+型血清肌酐、尿微量白蛋白排泄率高于Bg1Ⅱ+/-及Bg1Ⅱ-/-型(P<0.05);Bg1Ⅱ基因型各亚组间HOMA-IR、HQMA-β差异无统计学意义(P>0.05);(3)Bg1Ⅱ基因型各亚组间血脂水平差异无统计学意义(P>0.05)。结论α2β1整合素Bg1Ⅱ基因多态性与DN存在相关性,Bg1Ⅱ+等位基因可能是DN的危险因子之一。
Objective To study the association between α2β1 integrin gene polymorphism and DN. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of α2β1Bg1Ⅱ gene in 298 T2DM patients (182 with nephropathy, 116 without nephropathy) and 107 normal controls . Results (1) The genotype of Bg1Ⅱ + / + in DN group was higher than that in non-nephropathy group (DM group) and normal control group (NC group) (P <0.01). Compared with NC group, the frequency of Bg1Ⅱ + allele increased P <0.05). (2) The Bg1Ⅱ + / + serum creatinine and urinary albumin excretion rate of T2DM patients was higher than that of Bg1Ⅱ +/- and Bg1Ⅱ - / - type (P <0.05) -IR and HQMA-β had no statistical significance (P> 0.05). (3) There was no significant difference in serum lipids between subgroups of Bg1Ⅱ genotype (P> 0.05). Conclusion The polymorphism of α2β1 integrin Bg1 Ⅱ gene is correlated with the presence of DN. Bg1 Ⅱ + allele may be one of the risk factors of DN.