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【目的】观察龟板提取物诱导神经干细胞(neural stem cells,NSCs)向神经元细胞定向分化过程中相关micro RNA表达的变化。【方法】分离培养孕14 d SD胎鼠原代神经干细胞,经免疫荧光染色鉴定神经干细胞的特异抗原及其多向分化能力。神经干细胞随机设空白对照组、龟板提取物低浓度组(3μg/m L)、龟板提取物高浓度组(30μg/m L)。诱导分化7 d,提取各组细胞总RNA,采用实时荧光定量PCR法检测不同组别mi R-124和mi R-9的变化。【结果】与空白对照组比较,龟板提取物低浓度组mi R-124和mi R-9表达均无显著变化,龟板提取物高浓度组均显著升高(P<0.05)。【结论】龟板提取物可能通过调控mi R-124、mi R-9表达在NSCs向神经元细胞定向分化过程中起重要作用。
【Objective】 The purpose of this study was to observe the changes of microRNA expression in the course of the directional differentiation of neural stem cells (NSCs) to neurons. 【Method】 Primary neural stem cells from embryonic day 14 SD rats were isolated and cultured. The specific antigens of neural stem cells and their multipotential differentiation ability were identified by immunofluorescence staining. Neural stem cells were randomly assigned to a blank control group, a low concentration of turtle shell extract (3 μg / m L) and a high concentration of turtle shell extract (30 μg / m L). The cells were induced to differentiate for 7 days. Total RNA was extracted from each group. The changes of mi R-124 and mi R-9 in different groups were detected by real-time fluorescence quantitative PCR. 【Results】 Compared with the blank control group, the expression of mi R-124 and mi R-9 in the low concentration group of the turtle shell extract did not change significantly, and the high concentration group of the turtle shell extract increased significantly (P <0.05). 【Conclusion】 The extract of turtle shell may play an important role in the regulation of mi R-124, mi R-9 expression in NSCs to the differentiation of neurons.