以罗丹明B、水合肼、香草醛为原料合成了一种“off-on”型罗丹明B类席夫碱荧光探针,该探针在体积分数50%的乙腈溶液中对Cu2+具有良好的选择性。荧光滴定和Job’s实验表明,该探针与Cu2+形成1∶1的络合物,识别过程可逆。由该化合物对Cu2+响应的工作曲线得出:Cu2+浓度在0~10μmol/L内呈良好的线性关系,相关系数为r=0.997 2(n=5),检出限为0.48μmol/L。 Rhodamine B Schiff base fluorescent probe was synthesized from rhodamine B, hydrazine hydrate and vanillin. The probe has the following properties in the 50% acetonitrile solution: Good choice. Fluorescence titration and Job’s experiments show that the probe forms a 1: 1 complex with Cu2 +, and the recognition process is reversible. According to the working curve of Cu2 + response, Cu2 + showed a good linearity in the range of 0 ~ 10μmol / L with a correlation coefficient of 0.9972 (n = 5) and a detection limit of 0.48μmol / L.