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目的评价不同种类国产乙肝疫苗成人免疫效果,为制定免疫策略提供依据。方法采用酶联免疫法(ELISA)筛选免疫对象,按0、1、6免疫程序接种四种国产乙肝疫苗,接种完成一个月后双抗原夹心时间分辨免疫荧光分析法(IFM)定量检测抗-HBs。结果4种乙肝疫苗全程免疫者3 772人,平均抗-HBs阳性率为92.76%,其中男性90.9%,女性94.0%;大连高新、北京生物、深圳康泰、华北金坦4种疫苗免疫平均阳性率分别为89.8%、88.1%、97.0%、98.4%。疫苗免疫阳性率与年龄有统计学关联(P<0.001);免疫成功者抗-HBs GMT与疫苗种类相关,且随年龄增长呈下降趋势。结论4种乙肝疫苗成人免疫阳性率均较高,免疫效果良好,且免疫年龄越小效果越好。
Objective To evaluate the effect of different types of domestic hepatitis B vaccine on adult immunization and provide evidence for the development of immunization strategy. Methods Immunized mice were screened by enzyme-linked immunosorbent assay (ELISA). Four domestic hepatitis B vaccines were vaccinated according to 0, 1 and 6 immunization protocols. Anti-HBs were detected quantitatively by double antigen sandwich time-resolved immunofluorescence assay (IFM) . Results The total number of 4 HBsAg immunization was 3 772, the average positive rate of anti-HBs was 92.76%, including 90.9% for males and 94.0% for females; the average immunoprecipitation rates of 4 vaccines including Dalian Gaoxin, Beijing Bio, Shenzhen Kangtai, Respectively, 89.8%, 88.1%, 97.0%, 98.4%. The positive rate of vaccine immunization was statistically related to age (P <0.001). The anti-HBs GMT of immunized patients was correlated with the vaccine type and declined with age. Conclusions The positive rates of four kinds of hepatitis B vaccines are high in immunized adults, and the immune effect is good. The smaller the immunization age, the better the effect.