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目的:研究中药桃核承气改良方对家兔血管内皮损伤后STAT3(信号转导和转录活化因子)和VEGF(血管内皮生长因子)的影响。方法:健康新西兰大耳白兔40只,体重2~2.5kg,雌雄不拘。将动物分模型组(n=10)、中药组(n=10)、西药组(n=10)、空白组(n=10),模型组、中药组、西药组高酯饲料喂养4周后行腹主动脉内膜剥脱术造模。术后模型组、中药组、西药组正常饲料喂养,中药组予桃核承气改良方灌胃4周,西药组予舒降之、阿司匹林灌胃4周,模型组、空白组予生理盐水灌胃4周。采用半定量RT-PCR法测定血管中STAT3mRNA半定量和VEGF mRNA半定量。结果:模型组STAT3和VEGF mRNA半定量较空白组明显升高(P<0.05);西药组STAT3和VEGF mRNA半定量较模型组明显降低(P<0.05);中药组STAT3和VEGF mRNA半定量较模型组明显降低(P<0.05),中药组与西药组之间无明显差异(P>0.05)。结论:桃核承气改良方能防治血管内皮增生从而预防冠心病经皮冠状动脉介入治疗技术后冠脉再狭窄。
OBJECTIVE: To study the effects of the traditional Chinese medicine Taohe Chengqi reform prescription on STAT3 (signal transduction and activator of transcription) and VEGF (vascular endothelial growth factor) after vascular endothelial injury in rabbits. Methods: There were 40 healthy New Zealand white rabbits weighing 2 to 2.5 kg, either male or female. The animals were divided into model group (n=10), Chinese medicine group (n=10), western medicine group (n=10), and blank group (n=10). The model group, traditional Chinese medicine group, and western medicine group were fed with high-fat diet for 4 weeks. Aortic endarterectomy was performed. After the model group, the traditional Chinese medicine group and the western medicine group were fed with normal feed, the Chinese medicine group was premedicated with Taohe Chengqi modified prescription for 4 weeks, the western medicine group was given Shujiangzhi and aspirin for 4 weeks, and the model group and the blank group were given saline irrigation. Stomach 4 weeks. Semi-quantitative RT-PCR was used to determine semi-quantitative STAT3 mRNA and semi-quantitative VEGF mRNA in blood vessels. Results: Semiquantitative levels of STAT3 and VEGF mRNA in the model group were significantly higher than those in the blank group (P<0.05). Semiquantitative STAT3 and VEGF mRNA levels in the western medicine group were significantly lower than those in the model group (P<0.05); Semi-quantitative comparison of STAT3 and VEGF mRNA in the Chinese medicine group. The model group was significantly lower (P<0.05), and there was no significant difference between the Chinese medicine group and the western medicine group (P>0.05). Conclusion: Taohe Chengqi modified can prevent vascular endothelial hyperplasia and prevent coronary artery restenosis after percutaneous coronary intervention.