[方法]采用反相高效液相色谱法,使用C8 250 mm×4.6 mm(ID)不锈钢柱,以乙腈-0.1%磷酸水溶液(体积比65∶35)为流动相,流速为0.7 mL/min,在280 nm波长下,对啶磺草胺进行定量测定。[结果]方法的线性相关系数为0.999 9,定量检测限(LOQ)为230 mg/L,称量范围为240~4 000 mg/L,标准偏差为0.013 0,变异系数为0.177%,回收率为101.7%~102.4%。[结论]方法快速、准确,适用于啶磺草胺的定量分析。 [Method] The mobile phase consisted of C8 250 mm × 4.6 mm (ID) stainless steel column with acetonitrile - 0.1% phosphoric acid solution (65:35 by volume) at a flow rate of 0.7 mL / min using RP - HPLC. At 280 nm wavelength, the quantitative determination of sulfisalazine. [Result] The linear correlation coefficient was 0.999 9, LOQ was 230 mg / L, the weight range was 240-4000 mg / L, the standard deviation was 0.013 0, the coefficient of variation was 0.177%. The recovery 101.7% ~ 102.4%. [Conclusion] The method was rapid, accurate and suitable for the quantitative analysis of.