作者比较根尖肉芽肿中两种不同类型的多核巨细胞,异物型巨细胞和托通型巨细胞的形态,并检查它们在免疫组织化学上与巨噬细胞的类似性.作者选择5例根尖囊肿病例,2例可见托通型巨细胞;2例可见异物型巨细胞;还有1例二者都有,除 HE染色外,用未标记抗体过氧化物酶—抗过氧化物酶复合物的方法在5-μg 切片上显示溶菌酶,α_1-抗胰蛋白酶和α_1-抗凝乳胰蛋白酶的存在。组织切片脱蜡,用0.1%的胰蛋白酶在37℃下消化20分钟,在湿盒里用 The authors compared the morphology of two different types of multinucleated giant cells, foreign body giant cells, and Tonton giant cells in apical granuloma and examined their immunohistochemical similarity to macrophages. The authors selected five cases of roots. In case of cystic cysts, 2 cases showed giant Toto-type cells; 2 cases showed foreign body-type giant cells; and 1 case had both, and in addition to HE staining, they were compounded with unlabeled antibody peroxidase-antioxidant enzyme. The method of the material shows the presence of lysozyme, [alpha]_1-antitrypsin and [alpha]-1-antitrypsin on 5-μg sections. Tissue sections were deparaffinized and digested with 0.1% trypsin at 37°C for 20 minutes in a wet box