论文部分内容阅读
采用聚丙烯酰胺凝胶电泳对柑桔两个杂交组合克里迈丁×三湖化红、克里迈丁×宜昌橙F_1代杂种各30侏的GOT、SOD、POX、ME四种酶的同工酶进行了检测分析,发现在GOT—2、SOD—2、POX—2、ME—2四个酶位点上F_1代有分离,表明亲本在这些酶基因位点上处于杂合状态。GOT—2、SOD—2、POX—2的分离经x~2测验,符合孟德尔单基因遗传,与其相应的Got—2、Sod—2、Pox—2三个酶基因可作为遗传标记应用。ME—2的分离复杂,说明柑桔在遗传基础上十分复杂。
The results of polyacrylamide gel electrophoresis showed that GOT, SOD, POX and ME of two crosses of citrus were the same for the two crosses of CRIMMINTIN × SANHUHUHONG RED, CRIMETINTIN × YICHANG ORANGE F_1 hybrids The enzyme was detected and analyzed. It was found that the F_1 generation was separated at the four enzyme sites of GOT-2, SOD-2, POX-2 and ME-2, indicating that the parents were heterozygous at these enzyme loci. The isolates of GOT-2, SOD-2 and POX-2 were tested by x ~ 2 test and were consistent with Mendelian single-gene inheritance. The corresponding genes Got-2, Sod-2 and Pox-2 could be used as genetic markers. The separation of ME-2 is complex, indicating that citrus is very genetically complex.