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小麦幼苗经渐进水分胁迫处理后,叶绿体的电子传递活性将随处理时间逐渐的增长而降低.24h轻度胁迫下,光合全电子链、PSⅡ和PSⅠ电子传递活性有轻微下降,而48h中度和72h重度胁迫,将使它们明显降低.这表明,叶绿体电子传递活性对水分胁迫的响应与胁迫程度有关.PSⅡ的DCIP光还原活性与PSⅡ电子传递活性有相同的变化模式.类囊体膜和PSⅡ膜蛋白组分稳态水平的电泳分析表明,在中度和重度水分胁迫下,叶绿体光化学活性的降低与光合功能蛋白组分含量的减少有关.还讨论了植物光合电子传递功能对水分胁迫的敏感性
After progressive seedling stress, the electron transport activity of chloroplasts decreased with the gradual increase of treatment time. Under mild stress for 24h, electron transport activity of PSⅡ and PSⅠ in photosynthetic all-electron chain decreased slightly, while 48h moderate and 72h severe stress would make them significantly lower. This indicates that the response of chloroplast electron transport activity to water stress is related to the degree of stress. The photoreduction activity of PSII and PSⅡ electron transfer activity have the same change pattern. Electrophoretic analysis of the steady-state levels of thylakoid membrane and PS II membrane protein components showed that the decrease of chloroplast photochemical activity was related to the decrease of photosynthetic functional protein content under moderate and severe water stress. The sensitivity of plant photosynthetic electron transport to water stress is also discussed