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用含不同浓度（1，10，100，500，1000，5000ug／平皿）的无腺体棉仁（GCK）二甲亚砜（DMSO）提取物各0．1mL。对鼠伤寒沙门氏菌突变株（TA100，TA98，TA102，TA97）作Ames试验，结果表明试验组与阴性对照一致。取含40％（Ⅰ），20％（Ⅱ）GCK饲料和对照饲料（Ⅲ）喂养3个月的SD大鼠用环磷酰胺50mg／ks·d灌胃染毒的大鼠（阳性对照，Ⅳ）作骨髓细胞染色体畸变分析，结果表明试验鼠的骨髓细胞染色体畸变率与阴性对照（Ⅲ）一致（PM0．05），与阳性对照差异极显著（P＜0．001）。选用LACA小鼠，Ⅰ，Ⅱ组分别喂以未脱脂和脱脂的纯GCK，Ⅲ组喂以基础饲料为阴性对照，Ⅳ组用前法灌胃染毒并喂以见Ⅲ相同饲料作阳性对照，分组交配，作显性致死试验观察，结果试验鼠的致突变指数，5周内均在5以下与阴性对照一致，而阳性对照前三周就高达24．0，28．8和31．1。采用与染色体畸变分析试验相同的喂养条件分组，阳性对照（Ⅳ）改为1mg／kg·d敌枯双灌胃染毒，用SD大鼠作对胚胎毒性影响的致畸试验，结果表明，供试孕鼠的妊娠期情况与阴性对照一致，胎活仔率在95．7％以上，胚胎吸收率在5％以下。仔鼠发育正常，外观、内脏、骨骼无畸变现象。同样试验条件下的二代繁殖试验表明，孕鼠妊娠期情况、平均产仔数、仔鼠4d存活
0.1 mL of each dimethylsulfoxide (DMSO) extract of gland-free cottonseed (GCK) containing different concentrations (1, 10, 100, 500, 1000 and 5000 ug / plate) Ames test was conducted on Salmonella typhimurium mutants (TA100, TA98, TA102 and TA97). The results showed that the test group was consistent with the negative control. Sprague-Dawley rats were fed with cyclophosphamide (50mg / ks · d) for 3 months and fed with 40% (Ⅰ), 20% (Ⅱ) GCK feed and control feed (Ⅲ) ) For chromosomal aberrations analysis of bone marrow cells. The results showed that the chromosomal aberration rate of bone marrow cells in experimental mice was consistent with that of negative control (Ⅲ) (P> 0.05), which was significantly different from that of positive control (P <0.001). LACA mice were used. GroupsⅠandⅡwere fed with pure GCK without degreasing and degreasing. GroupⅢwas fed with basal diet as negative control. GroupⅣwas given intragastric administration with the same method as before, Grouping mating, for the dominant lethal test observation, the results of experimental mice mutagenicity index within 5 weeks were consistent with the negative control 5, while the positive control in the first three weeks up to 24.0,28.8 and 31.1. Using the same feeding conditions as the chromosomal aberration analysis group, the positive control (Ⅳ) was changed to 1mg / kg · d enemy enemy dry double gavage, with SD rat embryotoxicity teratogenicity test results showed that the test Gestational conditions in pregnant rats were the same as those in the negative controls. The rate of live births was above 95.7% and the rate of embryo resorption was below 5%. Offspring normal development, appearance, internal organs, skeletal no distortion. The same test conditions, the second generation of reproductive experiments showed that pregnant rats during pregnancy, the average number of littermate, offspring survival 4d