论文部分内容阅读
目的:建立以RP-HPLC法测定人血浆中齐多夫定浓度的方法。方法:色谱柱为Kromasil C18,流动相为0.5mol·L-1磷酸二氢钾溶液-甲醇(80∶20),流速为1.0mL·min-1,检测波长为267nm,灵敏度为0.002AUFS,柱温为室温。结果:齐多夫定和内标(氟康唑)的保留时间分别为8.1、5.7min;齐多夫定检测浓度在10～2000ng·mL-1范围内与峰面积积分值线性关系良好(r=0.9998),绝对回收率>97%,日内和日间RSD<9.4%;检测限和定量限分别为5、10ng·mL-1。结论:本法适用于齐多夫定血药浓度检测及临床药学研究。
Objective: To establish a method for the determination of zidovudine in human plasma by RP-HPLC. Methods: The chromatographic column was Kromasil C18 with a mobile phase of 0.5 mol·L-1 potassium dihydrogen phosphate solution-methanol (80:20) at a flow rate of 1.0 mL · min-1 at a detection wavelength of 267 nm with a sensitivity of 0.002 AUFS. The temperature is room temperature. Results: The retention times of zidovudine and internal standard (fluconazole) were 8.1 and 5.7 min, respectively. The calibration curve of zidovudine showed a good linear relationship with the integral of peak area in the range of 10 ~ 2000 ng · mL-1 = 0.9998). The absolute recovery was> 97%. The intraday and interday RSD were less than 9.4%. The limits of detection and quantification were 5 and 10 ng · mL -1, respectively. Conclusion: This method is suitable for zidovudine blood concentration testing and clinical pharmacy research.