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目的研究缺氧诱导丝裂原因子(hypoxia-induced mitogenic factor,HIMF)在先天性膈疝(congenital diaphragmatic hernia,CDH)胎鼠肺组织中的表达,探讨其在CDH肺发育不良中的作用。方法实验组10只BABL/C小鼠妊娠8 d时经胃管注入25 mg除草醚,正常对照组给予食用油,妊娠21 d行剖腹产,解剖胎鼠两侧肺组织,采用免疫组化、Western blot方法检测HIMF表达。结果实验组CDH致畸率56.5%,肺发育不良,处于假腺体期和原始肺小管期,HIMF蛋白显著表达下调(P<0.05);实验组内产生CDH者胎肺内HIMF表达水平与无CDH者比较差异无显著性意义(P>0.05)CDH膈疝侧与非膈疝侧肺组织HIMF表达差异无显著性意义(P>0.05)。结论在CDH肺发育不良组织中HIMF蛋白表达显著下调,且早于膈疝形成,可能参与CDH肺发育不良的发病机制。
Objective To investigate the expression of hypoxia-induced mitogenic factor (HIMF) in the lung tissue of congenital diaphragmatic hernia (CDH) fetal rat and to explore its role in CDH pulmonary dysplasia. Methods 10 BABL / C mice in experimental group were infused with 25 mg of weed-ether by gastric tube on the 8th day of pregnancy and edible oil in the normal control group. Caesarean section was performed on the 21st day of gestation. The lung tissues on both sides of the fetal rat were dissected. Immunohistochemistry, blot method to detect HIMF expression. Results In the experimental group, the CDH teratogenic rate was 56.5%, lung dysplasia was in the gingival stage and primary pulmonary tubule stage, HIMF protein was significantly down-regulated (P <0.05) CDH were no significant difference (P> 0.05) between the CDH diaphragmatic hernia and non-diaphragmatic hernia side of the HIMF expression was no significant difference (P> 0.05). Conclusions The expression of HIMF protein in CDH lung dysplasia was significantly down-regulated and earlier than diaphragmatic hernia formation, which may be involved in the pathogenesis of CDH hypoplasia.