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目的:研究逍遥散介导β-AR信号通路对于M2型巨噬细胞极化的调控。方法:在THP-1细胞培养模型上,观察NE对PMA、IL-4诱导的M2型巨噬细胞生成的影响,并观察逍遥散含药血清对该过程的影响,分别利用流式细胞技术、ELISA确定M2型巨噬细胞的生成,其中包括CD163、CD206、IL-12、IL-10的表达。结果:本研究发现NE处理后CD206、CD163、IL-10、cAMP表达均升高(P<0.05,P<0.01),而加入逍遥散含药血清后,CD163下降明显(P<0.05),CD206、IL-10、cAMP显著下降(P<0.01)。结论:NE能促进M2型巨噬细胞生成,而逍遥散能抑制NE处理后的M2细胞生成,逍遥散能通过抑制β-AR信号通路活化从而调控M2型巨噬细胞的生成。
Objective: To study the regulation of Xiaoyaosan-mediated β-AR signaling pathway on the polarization of M2 macrophages. Methods: The effect of NE on PMA and IL-4-induced M2 macrophage formation was observed in THP-1 cell culture model. The effects of Xiaoyao powder-containing serum on this process were observed. Flow cytometry, The formation of M2 macrophages was confirmed by ELISA, including expression of CD163, CD206, IL-12 and IL-10. Results: The levels of CD206, CD163, IL-10 and cAMP in NE-treated group were significantly higher than those in control group (P <0.05, P <0.01) , IL-10, cAMP decreased significantly (P <0.01). CONCLUSION: NE can promote the production of M2 macrophages. Xiaoyao powder can inhibit the generation of M2 cells after NE treatment. Xiaoyaosan can regulate the production of M2 macrophages by inhibiting the activation of β-AR signaling pathway.