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目的:建立高效液相色谱法同时测定肠激安方中芍药苷、木香烃内酯和去氢木香内酯3个有效成分的含量。方法:采用Phenomenex Luna C18色谱柱(250mm×4.6mm,5μm),以乙腈-水为流动相梯度洗脱,流速0.8mL·min-1,检测波长230nm,柱温30℃。结果:芍药苷、木香烃内酯和去氢木香内酯线性范围分别为0.15～3.03μg(r=0.9999),0.11～2.23μg(r=0.9999),0.10～2.03μg(r=0.9999);平均加样回收率(n=9)分别为100.9%,101.1%,102.0%。肠激安提取物中芍药苷、木香烃内酯和去氢木香内酯的含量分别为(3.38±0.05)mg·g-1,(1.66±0.03)mg·g-1,(1.87±0.02)mg·g-1。结论:本方法的精密度、稳定性、重复性和加样回收率均较好;所建立的HPLC方法准确、快速,可用于肠激安提取物中芍药苷、木香烃内酯和去氢木香内酯3个主要成分的含量测定。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of three effective components of paeoniflorin, geranolactone and dehydrocostuslactone. Methods: A Phenomenex Luna C18 column (250mm×4.6mm, 5μm) was used. The mobile phase was acetonitrile-water, and the flow rate was 0.8mL·min-1. The detection wavelength was 230nm and the column temperature was 30°C. Results: The linear ranges of peoniflorin, costunolide and dehydrocostuslactone were 0.15-3.03 μg (r=0.9999), 0.11-2.23 μg (r=0.9999), and 0.10-2.03 μg (r=0.9999), respectively. The average recovery (n=9) was 100.9%, 101.1%, and 102.0%, respectively. The contents of paeoniflorin, costunolide and dehydrocostuslactone in the extract of intestinal shock were (3.38±0.05) mg·g-1, (1.66±0.03) mg·g-1, (1.87±0.02), respectively. )mg g-1. Conclusion: The precision, stability, repeatability, and sample recovery of this method are all good. The established HPLC method is accurate and rapid. It can be used for extracts of intestines, paeoniflorin, woody lactone and dehydrogenated wood. Determination of the content of the three main components of the lactone.