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目的应用蛋白质组学方法研究弱精子症患者及正常生育男性精浆中的差异蛋白,发掘与弱精子症发生发展密切相关的蛋白质标志物。方法收集36例弱精子症患者和28例正常生育男性的精液样本,离心分离获取精浆,用H50蛋白质芯片结合蛋白质芯片-飞行时间质谱技术(SELDI-TOF-MS)对各组精浆样本进行检测,获取各样本的蛋白质指纹图谱,比较各组间蛋白质图谱表达差异。结果在相对分子质量在1 000~50 000Da范围内,共检测到239种有差异的蛋白峰,其中11种有统计学意义(P<0.05)。弱精子症组与正常生育男性精浆蛋白质谱相比,弱精子症组有8种差异蛋白质表达降低(P<0.05),其中M/Z为7867.33的蛋白质有显著差异性(P<0.01)。3种差异蛋白质M/Z为1247.54、1673.51、11158.2,较正常组表达增高,其中M/Z为1673.51的蛋白质有显著差异性(P<0.01)。结论弱精子症患者其精浆蛋白质群较正常生育男性精浆有显著改变,这些蛋白质的降低可能与精子活力受损有重要相关性,本研究对探究弱精子症的病因及其临床诊疗具有重要意义。
Objective To study the differential proteins in seminal plasma of patients with asthenospermia and normal fertility by using proteomics and to explore protein markers closely related to the occurrence and development of asthenospermia. Methods Semen samples of 36 patients with asthenospermia and 28 normal fertile men were collected and centrifuged to obtain seminal plasma. Seminal plasma samples were obtained from each group by H50 protein chip combined with time-of-flight mass spectrometry (SELDI-TOF-MS) Detection, access to the samples of the protein fingerprinting, protein expression differences between groups were compared. Results A total of 239 differential protein peaks were detected in the range of 1 000 ~ 50 000 Da, of which 11 were statistically significant (P <0.05). There were 8 differential protein expressions in asthenospermia group (P <0.05) compared with those in normal fertility group, and 7867.33 protein in M / Z group was significantly different (P <0.01). The three kinds of differential proteins M / Z were 1247.54, 1673.51 and 11158.2, which were higher than those in normal group. The proteins with M / Z 1673.51 were significantly different (P <0.01). Conclusions In patients with asthenospermia, the plasma protein level of the seminal plasma is significantly changed compared with that of normal male infertile men. The reduction of these proteins may be related to the impairment of sperm motility. This study is of great importance in exploring the etiology and clinical diagnosis of asthenospermia significance.