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目的观察缺血后处理对大鼠缺血再灌注心肌的保护作用,进一步探讨其心肌保护的作用机制。方法雄性Wistar大鼠随机分为3组:假手术组,缺血再灌注组,缺血后处理组,每组8只。建立在体心肌缺血再灌注模型,监测左室收缩压、左室舒张末压等心功能指标的变化;测定丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力。结果与假手术组比较,缺血再灌注组和缺血后处理组的心功能水平均显著降低,MDA含量显著增高,SOD活力显著下降(P<0.01);但与缺血再灌注组比较,缺血后处理组心功能恢复水平增高,SOD活力上升(P<0.05),MDA含量显著降低(P<0.01)。结论缺血后处理具有明确的心肌保护功能,可以改善心功能,减轻氧化应激损伤。
Objective To observe the protective effects of ischemic postconditioning on myocardial ischemia-reperfusion in rats and further explore the mechanism of myocardial protection. Methods Male Wistar rats were randomly divided into 3 groups: sham-operation group, ischemia-reperfusion group and ischemic postconditioning group, 8 rats in each group. Myocardial ischemia-reperfusion model was established to observe the changes of cardiac function indexes such as left ventricular systolic pressure and left ventricular end-diastolic pressure. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. Results Compared with the sham-operation group, the levels of cardiac function in ischemia-reperfusion group and ischemic postconditioning group were significantly decreased, MDA content was significantly increased and SOD activity was significantly decreased (P <0.01), but compared with ischemia reperfusion group, The ischemic postconditioning group had higher cardiac function recovery, increased SOD activity (P <0.05), and decreased MDA content (P <0.01). Conclusion Ischemic postconditioning has definite function of myocardial protection, which can improve cardiac function and reduce oxidative stress injury.