应用免疫组化 PAP 法对大鼠、家鸽、鳖、蟾蜍以及乌鳢的脊髓进行染色,观察 P 物质在这些动物脊髓中分布的情况,发现从低等到高等脊椎动物中,P物质在脊髓横切面中的分布位置基本相似,但在低等脊椎动物中阳性反应较弱,到高等脊椎动物中 P 物质有逐渐增多的趋势,动物在进化过程中对痛觉的敏感程度提高可能与此有关。 The spinal cords of rats, pigeons, cockroaches, cockroaches, and black locusts were stained with immunohistochemical PAP to observe the distribution of substance P in the spinal cord of these animals. It was found that substance P was in the spinal cord cross section from lower to higher vertebrates. The distribution locations are similar, but the positive response is lower in lower vertebrates and the P substance in higher vertebrates is gradually increasing. The sensitivity of animals to the development of pain sensitization may be related to this.