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目的 :通过对高功率脉冲微波 (HPPMW )辐照后Wistar大鼠血清中睾酮 (Testo)、雌二醇(E2 )、促甲状腺激素 (TSH)、游离甲状腺素T4 (FT4 )、皮质醇 (Cort)、醛固酮 (Ald)及生长激素 (HGH)的动态观察 ,以探讨HPPMW对内分泌系统的影响。方法 :二级雄性Wistar大鼠 35只 ,随机分为 7组 ,每组 5只 ,其中 1组为空白对照 ,其余 6组为辐照组。用高功率脉冲微波源 ,以功率密度为 30 5 0W/cm2 脉冲微波 (频率为 5 .4GHz ,脉宽为 2 6ns)辐照辐照组动物。于辐照后 1h ,6h ,2 4h ,7d ,14d及2 8d分别采血 ,用放射免疫法在FT630放免测定仪上进行统一测定。所有数据经SPSS8.0统计处理。结果 :大鼠被辐照后 ,其血清E2 在早期变化不明显 ,但在 14d时明显升高 (P <0 .0 1) ;Testo辐照后 1h和 14d明显下降 (P <0 .0 5 ) ;TSH于辐照后逐渐升高 ,14d到 2 8d时明显增高 (P <0 .0 5 ) ;FT4 辐照后 1h明显升高 (P <0 .0 5 )后逐渐降低 ,在 2 8d下降到最低点 (P <0 .0 1) ;Cort辐照后 1h即升高 ,14d达到峰值 (P <0 .0 5 ) ;Ald于照后 6h～ 14d略低于对照组 ,2 8d恢复 ;HGH辐照后明显低于照前水平 ,在 2 8d下降到最低点 (P <0 .0 5 )。结论 :HPPMW可引起大鼠血清激素水平的紊乱 ,既有早期影响 ,也表现为一定的持续效应。提示HPPMW可能
OBJECTIVE: To investigate the effect of HPPMW on testosterone, estradiol (E2), thyrotropin (TSH), free thyroxine T4 (FT4) and cortisol (Cortin) in Wistar rats after irradiation with high power pulsed microwave (HPPMW) ), Aldosterone (Ald) and growth hormone (HGH) were observed in order to investigate the effect of HPPMW on the endocrine system. Methods: Twenty-five male Wistar rats were randomly divided into 7 groups with 5 rats in each group. One group was blank control group and the other 6 groups were irradiation group. The animals were irradiated with high power pulsed microwave sources at a power density of 305 W / cm2 pulsed microwave (frequency of 5.4 GHz, pulse width of 26 ns). Blood samples were taken at 1h, 6h, 24h, 7d, 14d and 28d after irradiation, respectively, and radioimmunoassay was performed on the FT630 radioimmunoassay apparatus. All data by SPSS8.0 statistical processing. Results: Serum E2 did not change significantly in the early stage after irradiation in rats, but was significantly increased at 14 days (P <0.01). The levels of E2 at 1 and 14 d after Testo irradiation decreased significantly (P <0.05) ); TSH increased gradually after irradiation and increased significantly from 14d to 28d (P <0.05); FT4 decreased significantly at 1h (P <0.05) (P <0.01). Cort increased at 1h and peaked at 14d (P <0.05). Ald was slightly lower than control group 6h-14d after irradiation and resumed at 28d ; HGH after irradiation was significantly lower than the pre-irradiation level, dropping to the lowest point on 28d (P <0.05). Conclusion: HPPMW can cause disturbance of serum hormone level in rats, which has both early and sustained effects. Prompt HPPMW possible