应用改良CTAB法分别提取生长于珠海和海南的3种药用红树植物秋茄、老鼠簕和桐花树的基因组DNA,以真核生物rDNA ITS区的通用引物分别对其rDNA ITS区进行nPCR扩增及测序,并对测序结果进行对位排列。结果表明,不同来源的药用红树植物的rDNA ITS序列上存在扩增碱基差异,rDNA ITS测序结果可作为鉴定药用红树植物种质资源的分子标记之一。 The genomic DNAs of three species of Medicinal mangroves, Candel spp. And Aegiceras corniculatum, which were grown in Zhuhai and Hainan, were extracted by modified CTAB method. The rDNA ITS regions were amplified by nPCR Increase and sequencing, and the alignment of the results of sequencing. The results showed that rDNA ITS sequences of medicinal mangrove plants from different origins had amplification base differences, and the results of rDNA ITS sequencing could be used as one of the molecular markers for identification of medicinal mangrove germplasm resources.