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目的探讨脉冲外电磁场协同表阿霉素(EPI)-羧甲基葡聚糖氧化铁磁性纳米颗粒(CDMN)对人膀胱癌BIU-87细胞体外增殖活性和裸鼠皮下移植膀胱癌生长及其凋亡的影响。方法用化学共沉淀和氧化还原法制备EPI-CDMN并检测其理化性质,建立膀胱癌BIU-87细胞裸鼠皮下移植瘤动物模型,并设立空白对照组、磁场组、EPI组和EPI-CDMN组作为对照。分别采用噻唑蓝(MTT)比色法、双标流式细胞术、DNA原位末端标记法观察脉冲外电磁场联合EPI-CDMN对BIU-87细胞生长增殖活性和裸鼠皮下瘤生长状况及凋亡的影响。结果EPI-CDMN的直径、比饱和磁化强度分别为8～10 nm、0．22 emu/g。外磁场协同EPI-CDMN可以显著抑制BIU-87细胞增殖,抑制率达(21．82±3．18)%,并诱导细胞发生明显的凋亡,凋亡率为(16．8±3．4)%,均显著高于其他各组(P＜0．05)；裸鼠皮下瘤体积(1．57±0．42)cm~3和重量(2．00±0．21)g,均显著低于其他各组,抑瘤率51．69%和细胞凋亡指数(60．45±6．93)%均明显高于其他各组。结论外磁场可以显著增强EPI-CDMN对膀胱肿瘤的杀伤作用,为抗膀胱肿瘤的磁靶向治疗提供了实验依据。
OBJECTIVE To investigate the in vitro proliferation of human bladder cancer cell line BIU-87 and the growth of bladder cancer in nude mice subcutaneously implanted with epirubicin (EPI) -carboxymethyl dextran iron oxide magnetic nanoparticles (CDMN) The impact of death. Methods EPI-CDMN was prepared by chemical coprecipitation and oxidation-reduction method. The physical and chemical properties of EPI-CDMN were assayed. The animal model of bladder cancer BIU-87 cells transplanted subcutaneously was established and the blank control group, magnetic field group, EPI group and EPI-CDMN group as comparison. The growth and proliferation of BIU-87 cells and the growth and apoptosis of BIU-87 cells in nude mice were observed by MTT colorimetric assay, double-labeled flow cytometry and DNA in situ end labeling. Impact. Results The diameter and specific saturation magnetization of EPI-CDMN were 8-10 nm and 0.22 emu / g, respectively. EPI-CDMN could significantly inhibit the proliferation of BIU-87 cells with the inhibition rate of (21.82 ± 3.18)%, and induce significant apoptosis in BIU-87 cells with the apoptosis rate of (16.8 ± 3.4) ) Were significantly higher than those in other groups (P <0.05). The volume of hypodermal tumor in nude mice was (1.57 ± 0.42) cm ~ 3 and the weight was (2.00 ± 0.21) g, Lower than the other groups, the tumor inhibition rate of 51.69% and apoptosis index (60.45 ± 6.93)% were significantly higher than the other groups. Conclusion External magnetic field can significantly enhance the killing effect of EPI-CDMN on bladder tumor and provide an experimental basis for magnetic targeted therapy of bladder cancer.