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目的测定毛蕊花糖苷的油水分配系数。方法采用紫外分光光度法测定毛蕊花糖苷的浓度,结合摇瓶法,正辛醇-水系统测定毛蕊花糖苷的油水分配系数。结果在0~30μg/mL范围内,毛蕊花糖苷的浓度与吸光值成线性关系,相关系数为0.999;平均回收率为(102.16±0.703)%,RSD为0.688%(n=3);油水分配系数为0.273,logP=-0.566(n=3),说明毛蕊花糖苷易溶于水。结论紫外分光光度法测定毛蕊花糖苷浓度操作简便,准确性好,可作为毛蕊花糖苷油水分配系数测定的方法。
Objective To determine the partition coefficient of verbascoside. Methods The concentration of verbascoside was determined by ultraviolet spectrophotometry. The oil-water partition coefficient of verbascoside was determined by shake flask method and n-octanol-water system. Results The concentrations of verbascoside in the range of 0 ~ 30μg / mL showed a linear relationship with the absorbance. The correlation coefficient was 0.999. The average recovery was (102.16 ± 0.703)% and the RSD was 0.688% (n = 3) 0.273, logP = -0.566 (n = 3), indicating that verbascoside soluble in water. Conclusion The determination of verbascoside by UV spectrophotometry is simple, accurate and can be used as a method for the determination of verbascoside oil-water partition coefficient.