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目的探讨高复发膀胱移行细胞癌基因组非平衡性的变化,寻找与膀胱癌复发相关的染色体位点或区段的畸变。方法选择20例膀胱移行细胞癌患者的石蜡包埋组织标本,经随访分为高复发组11例,无复发组9例。应用比较基因组杂交(CGH)对每一个标本进行检测,得出其染色体畸变的位点或区段,应用χ2确切概率法统计高复发组与无复发组畸变的差异。结果增益频率较高的染色体区段或位点位于1p、1q、3p、5p、5q、8q、17q;缺失频率较高的染色体区段或位点位于4q、8p、9q、11q、11p、17p。高复发组与无复发组相比较,9q、11p缺失的差异有统计学意义(P<0.05);而9q、11p同时缺失的差异无统计学意义(P>0.05)。结论染色体9q、11p上某些区段或位点的缺失与膀胱癌复发有关,此2个染色体臂上可能存在膀胱移行细胞癌的“复发相关基因”,其上的基因缺失或失活可能导致膀胱移行细胞癌的复发。
Objective To investigate the changes of the genomic imbalance in high recurrence of bladder transitional cell carcinoma and to find out the aberrations of chromosomal loci or segments related to the recurrence of bladder cancer. Methods Twenty paraffin-embedded tissue samples from patients with bladder transitional cell carcinoma were selected and divided into 11 cases of high recurrence group and 9 cases of non-recurrence group. Each sample was detected by comparative genomic hybridization (CGH), and the site or segment of chromosomal aberrations was obtained. The difference of the aberrations between the high-recurrence group and the non-recurrence group was calculated by χ2 exact probability method. Results The chromosome segments or loci with higher gain frequency were located at 1p, 1q, 3p, 5p, 5q, 8q and 17q. The chromosome segments or sites with higher deletion frequency were located at 4q, 8p, 9q, 11q, 11p and 17p . The difference of 9q and 11p deletion between high recurrence group and no recurrence group was statistically significant (P <0.05), while no significant difference was found between 9q and 11p deletion (P> 0.05). Conclusion The deletion of some segments or loci on chromosomes 9q and 11p is related to the recurrence of bladder cancer. There may be “relapse-related genes” in bladder transitional cell carcinoma on these two chromosome arms. The gene deletion or inactivation may cause Recurrence of bladder transitional cell carcinoma.