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目的 研究食管癌组织的CD_(44V) mRNA表达及其临床意义。方法 应用RT-PCR及Southern blot方法研究了48例食管癌及4例正常食管组织中CD_(44V)mRNA表达。结果 食管癌组织的CD_(44V)mRNA阳性表达率为83.3％(40/48),4例正常食管组织均未检测到CD_(44V)mRNA表达,统计学分析显示CD_(44V)mRNA阳性表达与食管癌的病理分级、临床分期以及淋巴结转移是显著相关性(P<0.05)。结论 CD_(44V)mRNA表达可作为食管癌恶性程度的一个标志和估价其预后的指标。
Objective To study the expression of CD_(44V) mRNA in esophageal carcinoma and its clinical significance. Methods RT-PCR and Southern blot were used to study the expression of CD_(44V) mRNA in 48 cases of esophageal cancer and 4 normal esophageal tissues. Results The positive expression rate of CD44V mRNA in esophageal cancer tissue was 83.3% (40/48). No CD44V mRNA expression was detected in 4 normal esophageal tissues. The positive expression of CD44V mRNA was detected by statistical analysis. The pathological grade, clinical stage and lymph node metastasis of esophageal cancer were significantly correlated (P<0.05). Conclusion The expression of CD_(44V) mRNA can be used as a marker of malignant degree of esophageal cancer and an index to evaluate its prognosis.