论文部分内容阅读
目的 :研究吲哚美辛胃损伤中内皮素 (ET)、一氧化氮 (NO)、氧自由基的作用 ,以及胃动力对这种损伤的影响。方法 :雄性DS大鼠随机分 4组 :对照组、吲哚美辛 5mg/kg组、吲哚美辛 2 5mg/kg组、阿托品组 (阿托品 1mg/kg +吲哚美辛 2 5mg/kg)。吲哚美辛灌胃 ,灌胃前 10min阿托品皮下注射。取动脉血测ET、NO、丙二醛 (MDA)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)水平 ,进行胃形态学观察。结果 :5mg/kg吲哚美辛不引起胃粘膜损伤 ,各检测指标与对照组无差异 ;2 5mg/kg吲哚美辛可引起胃粘膜显著出血性损伤 ,损伤指数为 38 5 7± 12 4 7,病理损伤积分为13 36± 3 37;ET 1和MDA水平升高 (P <0 0 1) ,NO、SOD、GSH Px水平降低 (P <0 0 1,P <0 0 1,P <0 0 5 ) ;阿托品组粘膜损伤较轻 ,损伤指数为 8 71± 3 35 ,病理损伤积分为 3 77± 1 0 4,ET 1含量和对照组无差异 ,MDA水平升高 (P <0 0 5 ) ,NO、SOD含量有所下降 (P <0 0 5 ) ,GSH Px含量无变化。结论 :吲哚美辛所致胃粘膜损伤中 ,ET 1和MDA生成增加起损害作用 ,内源性NO、SOD和GSH Px可清除氧自由基 ,有保护作用 ;阿托品对吲哚美辛所致胃粘膜损伤的保护作用 ,提示胃动力增加在吲哚美辛致溃疡中有重要作用。
Objective: To study the effect of endothelin (ET), nitric oxide (NO) and oxygen free radical in indomethacin gastric injury and the effect of gastric motility on this injury. Methods: Male DS rats were randomly divided into 4 groups: control group, indomethacin 5mg / kg group, indomethacin 25mg / kg group, atropine group (atropine 1mg / kg + indomethacin 25mg / kg) . Indomethacin intragastric administration, 10 minutes before the administration of atropine subcutaneous injection. The blood levels of ET, NO, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH Px) were measured and the morphology of gastric mucosa was observed. Results: Indometacin 5mg / kg did not cause gastric mucosal injury, and there was no difference between the test and control groups. Indometacin 25mg / kg could cause significant hemorrhagic injury in gastric mucosa with the injury index of 38 57 ± 12 4 7, the pathological injury score was 13 36 ± 3 37; the levels of ET 1 and MDA increased (P <0.01), the levels of NO, SOD and GSH Px decreased (P <0.01, P <0.01, P < 0 05). The ametropine group showed less mucosal lesion with an injury index of 8 71 ± 3 35 and a pathological injury score of 3 77 ± 1 0 4. There was no difference in ET 1 content between the control group and the MDA level (P <0 0 5), NO, SOD decreased (P <0 05), GSH Px content did not change. CONCLUSION: In the gastric mucosal injury induced by indomethacin, ET 1 and MDA may play an important role in the injury. Endogenous NO, SOD and GSH Px may scavenge oxygen free radicals, which may be protective. Indomethacin induced by atropine The protective effect of gastric mucosal injury, suggesting that increased gastric motility indometacin caused ulcer in an important role.