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本文采用体重２００±２０ｇ健康雄性Ｗｉｓｔａｒ大鼠，随机分为平原冻伤（ＦＮ）组、急性缺氧冻伤（ＦＡＨ）组和缺氧习服缺氧冻伤（ＦＨＡＣ）组，实验观察了大鼠右后肢重度冻伤前后各组大鼠双后肢皮肤微循环灌流量的改变。结果表明，平原冻伤使大鼠双后肢微循环灌流量明显减少，提示局部重度冻伤对微循环的影响不只局限于冻区也涉及到对侧肢体。冷冻前ＦＡＨ组大鼠微循环灌流量已明显低于ＦＮ组，表明急性缺氧时血容量进行代偿性的再分配，使微循环灌流量减少；ＦＡＨ组大鼠冻后双后肢微循环灌流量的改变结果提示急性缺氧可加重其冻伤对微循环的损伤程度。ＦＨＡＣ组大鼠冻前微循环灌流量非常明显地低于正常对照，也明显低于急性缺氧对照，表明缺氧习服可造成微循环障碍；ＦＨＡＣ组大鼠冻肢微循环灌流量非常明显地低于ＦＮ组，提示缺氧习服加重高原冻伤引起的微循环障碍。
In this study, healthy male Wistar rats weighing 200 ± 20g were randomly divided into three groups: FN group, FAH group and hypoxic-hypoxic-frost injury group. Changes of skin and microcirculation perfusion in both hindlimbs of rats before and after severe frostbite. The results showed that plain frostbite caused a significant decrease of microcirculation perfusion in both hindlimbs, suggesting that the effect of local severe frostbite on microcirculation is not limited to the frozen zone but also to the contralateral limbs. The microcirculation perfusion of rats in FAH group was significantly lower than that in FN group before freezing, indicating compensatory redistribution of blood volume in acute hypoxia, and decreased microcirculation perfusion; Changes in flow rate results suggest that acute hypoxia may aggravate the degree of injury to the microcirculation of frostbite. The microcirculation perfusion of FHAC group was obviously lower than that of normal control group and also lower than that of acute hypoxia control group, which indicated that microcirculation disturbance could be caused by hypoxic preconditioning. The perfusion of frozen limb microcirculation in FHAC group was very obvious Lower than the FN group, suggesting hypoxia accustomed to aggravate plateau frostbite caused by microcirculation.