论文部分内容阅读
作者等用自副沙眼分离的E型7RIC衣原体做为研究材料,在McCoy细胞内培养繁殖,对其发育周期进行了电镜及光学显微镜观察。方法是:McCoy细胞长成单层时加入适量的CytochalasiaB,然后接种TRIC衣原体,在33℃3,000g离心1小时,置35℃暖箱培养,定期取样检查。作者等认为TRIC衣原体在细胞培养中只完成一个发育周期,而无第二周期。电镜观察是将接种感染的细胞做成切片,用醋酸铀酰及枸隙酸铅双染,以Philips EM201电镜观察。光学显微镜检查时则用姬氏、碘、荧光抗体及吖啶橙等染色方法。为观察一步生长曲线,将接种的细胞培养自0～96小时内不同时期的样品收获冻存,分别滴定其细胞、培养液以及二者合并的感染滴度。测定感染滴度是将样品再接种于经Cytochalasitt B
The authors used Chlamydia trachomatis E isolated from Parallax as a research material, cultured and propagated in McCoy cells, and observed its developmental cycle by electron microscopy and optical microscopy. Methods: McCoy cells grow monolayer by adding appropriate amount of CytochalasiaB, and then inoculated with TRIC Chlamydia, centrifuged at 3,000g at 33 ℃ for 1 hour, 35 ℃ incubation incubator, regular sampling. The authors believe that TRIC Chlamydia in cell culture to complete only one development cycle, but no second cycle. Electron microscopy was performed inoculation of infected cells into sections, uranyl acetate and lead citrate double staining, electron microscopy to Philips EM201. Light microscopy with Ji, iodine, fluorescent antibodies and acridine orange and other staining methods. To observe the one-step growth curve, the inoculated cells were harvested from 0 to 96 hours in different periods and frozen. The infected cells were titrated with the culture medium and the combined infection titer. Infection titers were determined by re-inoculation of samples with Cytochalasitt B