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测定总辅酶A的效价,一般采用磺胺乙酰化法,即以乙酸盐作为乙酰基供体,以磺胺作为受体,以腺三磷(ATP)为激活剂,在鸽肝乙酰化酶的作用下,辅酶A能定量地将乙酰基转移在磺胺上,使成乙酰磺胺。剩下没有被乙酰化的磺胺,经亚硝酸钠重氮化后,与萘乙二胺形成淡红色偶氮化合物的溶液,在545nm波长处测定吸收度,即可直接计算乙酰化的磺胺量。当用已知活性单位辅酶A的标准品作为对照时,则可计算出供试品中辅酶A的活性单位。
Determination of the total coenzyme A titer, the general use of sulfonamidisation method that acetate as an acetyl donor, sulfanilamide as a receptor, adenosine triphosphate (ATP) as an activator in pigeon liver acetylase Under the action of coenzyme A can quantitatively transfer the acetyl group in the sulfonamide, making into acesulfame. The remaining non-acetylated sulfonamides, diazotized by sodium nitrite, and naphthylethylenediamine to form a reddish azo compound solution, absorbance was measured at a wavelength of 545nm, you can directly calculate the amount of acetylated sulfonamide. When using the standard of the known activity unit Coenzyme A as a control, the activity unit of coenzyme A in the test product can be calculated.