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本文采用正常人外周血2～4m1,1000～1500r/min离心10分钟,4%戊二醛固定后按常规电镜标本制作。半薄切片定位白细胞,制超薄切片收集于无膜镍网上。超薄切片先经H_2O_2蚀刻,继之用1%卵蛋白处理15分钟,然后切片依次孵育于抗人铁蛋白抗体(一种为兔抗人铁蛋白多克隆抗体,另一种为鼠抗人铁蛋白单克隆抗体)、10nm葡萄球菌A蛋白-胶体金探针(PAG)中,最后经醋酸铀和柠檬酸铅复染,JEM-1200EX透射电镜观察。结果如下:各种血细胞的超微结构均清晰可见,两种抗体在白细胞内的反应显著不同。经心脏铁蛋白单克隆抗体染色后,发现阳性胶体金颗粒只存在于嗜酸性粒细胞内的颗粒上,细
In this paper, normal human peripheral blood 2 ~ 4m1, 1000 ~ 1500r / min centrifugation for 10 minutes, 4% glutaraldehyde fixed by conventional electron microscopy specimens. Semi-thin sections locate leukocytes and make ultrathin sections collected on a nickel-free membrane. Ultrathin sections were first etched with H 2 O 2, followed by 1% ovalbumin for 15 minutes and then sectioned sequentially in anti-human ferritin antibody (one rabbit anti-human ferritin polyclonal antibody and the other mouse anti-human iron Protein monoclonal antibody), 10 nm staphylococcus a protein - colloidal gold probe (PAG), and finally stained with uranyl acetate and lead citrate, and observed with a transmission electron microscope JEM-1200EX. The results are as follows: The ultrastructures of various blood cells are clearly visible, and the responses of the two antibodies in leukocytes are significantly different. After staining with cardiac ferritin monoclonal antibody, it was found that the positive colloidal gold particles only existed on the granules of eosinophils