【摘 要】
:
介绍骨髓粒-单系祖细胞(CFU-GM)集落姊妹染色单体互换(SCE)的制备方法,应用时应注意集落细胞的收集,Brdu及秋水仙胺浓度和作用时间等问题,为临床细胞遗传学的研究提供了新的手段。本文用本法删定27例
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介绍骨髓粒-单系祖细胞(CFU-GM)集落姊妹染色单体互换(SCE)的制备方法,应用时应注意集落细胞的收集,Brdu及秋水仙胺浓度和作用时间等问题,为临床细胞遗传学的研究提供了新的手段。本文用本法删定27例健康成人骨髓CFU-GM集落染色体SCE频率为4.59±0.67次/集落细胞,该值可作为正常人分髓CFU-GM集落SCE的正常值供临床和实验参考。
This article introduces the preparation method of colony-sister chromatid exchange (CFU-GM) of bone marrow granulocyte-monocytic progenitor cells (CFU-GM) .It should pay attention to the collection of colony cells, concentration of Brdu and colchicines and the action time, Cytogenetics provides a new means of research. In this study, the frequency of SCE was determined in 27 CFU-GM colonies from healthy adults with a mean value of 4.59 ± 0.67 cells / colony, which could be used as a normal and normal SCU for normal human CFU-GM colonies for clinical and Experimental reference.
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