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采用无机磷合成培养基从大豆根际分离到223株细菌,其中能够产生明显溶磷圈的菌株47株,且在土壤中添加1%Ca3(PO4)2有利于筛选获得具有高效溶磷能力的菌株。对菌株的溶磷量进行测定,测得菌株的溶磷量为51.64~199.20 mg·L-1,对溶磷能力最强的菌株SR95进行平板传代,并不断提高培养基中Ca3(PO4)2含量,可使菌株的溶磷量从199.20 mg·L-1提高到243.71 mg·L-1。对分离到溶磷能力较强的13株菌进行16S rDNA部分序列测定,经在线比对并构建系统发育树,发现其分布在7个不同种当中,其中溶磷能力最强的菌株SR95为成团泛菌,这在以往研究中未见报道。
In total, 223 bacterial strains were isolated from soybean rhizosphere by inorgano-phosphorus media, of which 47 strains were able to produce obvious phosphate-solubilizing bacteria. The addition of 1% Ca3 (PO4) 2 to the soil was favorable for the screening of highly soluble phosphorus Strain. The amount of phosphate solubilizing in the strain was measured. The amount of phosphate solubilizing strain was 51.64-199.20 mg · L-1, and the strain SR95 with the highest ability to dissolve phosphorus was plate-passed, and the content of Ca3 (PO4) 2 , The amount of phosphate solubilizing bacteria could be increased from 199.20 mg · L-1 to 243.71 mg · L-1. The 16S rDNA partial sequences of 13 strains with strong ability of dissolving phosphorus were determined. Through online comparison and construction of phylogenetic tree, it was found that they were distributed in seven different species. The strain SR95 with the strongest ability of dissolving phosphorus was Pantoea agglomerans, which has not been reported in previous studies.